R2C2 full-length cDNA sequencing of hemoglobin depleted polar bear wh... - SRA

SRX5243180: R2C2 full-length cDNA sequencing of hemoglobin depleted polar bear whole blood transcriptome
1 OXFORD_NANOPORE (MinION) run: 60,986 spots, 260.4M bases, 204.1Mb downloads

Design: R2C2 protocol. Full-length cDNA generated using oligodT priming and template switching, depleted of hemoglobin transcripts using Long-DASH protocol, and circularized using Gibson Assembly, amplified using rolling circle amplification, debranched.

Submitted by: (UCSC)

Study: Hemoglobin depleted polar bear whole blood transcriptome

PRJNA514749 • SRP178553 • All experiments • All runs

show Abstracthide Abstract

Smart-seq2 Illumina and R2C2 ONT data for polar bear transcripts. Input full-length cDNA was depleted of hemoglobin using Long-DASH method.

Sample:

SAMN10734297 • SRS4245857 • All experiments • All runs

Organism: Ursus maritimus

Library:

Name: PB19_depleted_R2C2_R1

Instrument: MinION

Strategy: FL-cDNA